sphi hf Search Results


sphi hf  (New England Biolabs)
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New England Biolabs sphi hf
Genetic confirmation of complementation <t>of</t> <t>senX3</t> ::Tn and regX3 ::Tn. (A) . Diagram of a 2824-bp fragment containing the senX3-regX3 operon from the genome of wild-type CDC1551. (B) FseI digestion of genomic DNA and hybridization with dig-labeled senX3 probe reveals fragments measuring 3.7-kb and 5.7-kb in CDC1551 and senX 3::Tn, respectively, as well as 5.7-kb and 7.7-kb fragments in senX3 ::Tn Comp. (C) <t>SphI</t> digestion of genomic DNA followed by hybridization with dig-labeled regX3 probe reveals 5.5-kb and 7.5-kb fragments in CDC1551 and regX3 ::Tn, respectively, as well as 7.5-kb and 4.6-kb fragments in regX3 ::Tn Comp. (D) Southern analysis of senX3 complementation. Lanes 1 and 2: SenX3 ::Tn Comp candidates with appropriately-sized bands; Lane 3: SenX 3::Tn; Lane 4: Wild-type CDC1551. (E) Southern analysis of regX3 complementation. Lane 1: Wild-type CDC551; Lane 2: regX 3::Tn; Lanes 3–5: regX3 ::Tn Comp candidates with appropriately-sized bands.
Sphi Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genetic confirmation of complementation of senX3 ::Tn and regX3 ::Tn. (A) . Diagram of a 2824-bp fragment containing the senX3-regX3 operon from the genome of wild-type CDC1551. (B) FseI digestion of genomic DNA and hybridization with dig-labeled senX3 probe reveals fragments measuring 3.7-kb and 5.7-kb in CDC1551 and senX 3::Tn, respectively, as well as 5.7-kb and 7.7-kb fragments in senX3 ::Tn Comp. (C) SphI digestion of genomic DNA followed by hybridization with dig-labeled regX3 probe reveals 5.5-kb and 7.5-kb fragments in CDC1551 and regX3 ::Tn, respectively, as well as 7.5-kb and 4.6-kb fragments in regX3 ::Tn Comp. (D) Southern analysis of senX3 complementation. Lanes 1 and 2: SenX3 ::Tn Comp candidates with appropriately-sized bands; Lane 3: SenX 3::Tn; Lane 4: Wild-type CDC1551. (E) Southern analysis of regX3 complementation. Lane 1: Wild-type CDC551; Lane 2: regX 3::Tn; Lanes 3–5: regX3 ::Tn Comp candidates with appropriately-sized bands.

Journal: BMC Microbiology

Article Title: senX3 -independent contribution of regX3 to Mycobacterium tuberculosis virulence

doi: 10.1186/s12866-014-0265-8

Figure Lengend Snippet: Genetic confirmation of complementation of senX3 ::Tn and regX3 ::Tn. (A) . Diagram of a 2824-bp fragment containing the senX3-regX3 operon from the genome of wild-type CDC1551. (B) FseI digestion of genomic DNA and hybridization with dig-labeled senX3 probe reveals fragments measuring 3.7-kb and 5.7-kb in CDC1551 and senX 3::Tn, respectively, as well as 5.7-kb and 7.7-kb fragments in senX3 ::Tn Comp. (C) SphI digestion of genomic DNA followed by hybridization with dig-labeled regX3 probe reveals 5.5-kb and 7.5-kb fragments in CDC1551 and regX3 ::Tn, respectively, as well as 7.5-kb and 4.6-kb fragments in regX3 ::Tn Comp. (D) Southern analysis of senX3 complementation. Lanes 1 and 2: SenX3 ::Tn Comp candidates with appropriately-sized bands; Lane 3: SenX 3::Tn; Lane 4: Wild-type CDC1551. (E) Southern analysis of regX3 complementation. Lane 1: Wild-type CDC551; Lane 2: regX 3::Tn; Lanes 3–5: regX3 ::Tn Comp candidates with appropriately-sized bands.

Article Snippet: Genomic DNA from senX3 ::Tn and regX3 ::Tn complement candidates was digested with FseI and SphI-HF (New England Biolabs), respectively, and electrophoresis was performed on 1% agarose gels.

Techniques: Hybridization, Labeling